【摘要】 目的　探索曼氏血吸虫吡喹酮抗性的遗传性状和传播规律。方法　以 W1特异性序列为引物 ,采用直接 PCR技术鉴别出单性克隆尾蚴的性别 ;用曼氏血吸虫吡喹酮抗性株和敏感株单性尾蚴感染远交系 CD1 鼠 ,进行血吸虫抗性株与敏感株的杂交实验 ;采用体外虫卵、毛蚴和尾蚴吡喹酮敏感性检测法 ,评价 F1 和 F2 代虫体吡喹酮抗性水平。结果　吡喹酮抗性株和敏感株杂交 F1 和 F2代均表现出抗性株特征。结论　曼氏血吸虫吡喹酮抗性性状可能系显性遗传更多还原

【Abstract】 Objective To determine the genetic basis of the resistance of Schistosoma mansoni to praziquantel and understand whether the resistance is dominant or recessive trait. Methods A schistosome cross was undertaken between a praziquantel-susceptible and a praziquantel-resistant isolate by using infections of single-sex cercariae which were identified by a direct W1-specific PCR technique. The resistance of F 1 and F 2 generation to praziquantel were evaluated by using in vitro egg, miracidial and cercarial tests. Results The F 1 hybrid progeny from cross between the the susceptible and the resistant isolates were resistant to praziquantel. The resistant phenotype reappeared in the F 2 progeny. Conclusions The praziquantel resistance behavior may like a dominant trait.更多还原