【摘要】 目的 探讨低温干预起始时间对新生大鼠缺氧缺血性脑损伤的影响。方法 Wistar 7日龄新生大鼠,随机分到:缺氧缺血后0 min、60 min、120 min、6 h起始亚低温干预组、常温(37℃)恢复组、正常对照组(假结扎组),每组30只。每组20只于缺氧缺血后78 h处死,10只用于测定脑组织含水量,10只经灌注固定,冠状切片进行微管相关蛋白-2(MAP-2)免疫组化染色,测定脑梗塞面积;另外10只于生后42d用迷宫实验检测其学习和记忆能力。结果 (1)亚低温干预各组大鼠脑组织含水量与常温恢复组相比显著减少(P<0.05);(2)亚低温干预各组大鼠脑梗塞面积与常温恢复组相比显著降低(P<0.05),且随着亚低温干预起始时间的延迟,梗塞面积逐渐增大;延迟6 h的干预仍有效;(3)亚低温干预各组大鼠学习和记忆能力显著高于常温恢复组(P<0.05)。结论亚低温干预效果与缺氧缺血后低温的起始时间有关,延迟6 h的亚低温干预仍有效。更多还原

【Abstract】 To study effect of hypothennia at different start time on neonatal rats hypoxic - ischemic brain damage. Methods Seven - day - old neborn Wistar rats were randomized to groups: including started at 0 min, 60 min, 120 min, 6 h hypothennia group and nonnothermic group (37℃) after hypoxi-cischemic, control group (Sham - ligated group) . There were 30 rat pups in each group. 20 Pups of each group were sacrificed at 78 h for measurements for brain water content or histology examination of MAP -2 immunohistochemistry. 10 Pups of each group were trained and tested at P42 using Y - radial maze stimulator for measurement of study and memory ability. Results (1) The content of brain water decreased significantly in hypothennia groups compared with normothermic group ( P<0.05); (2) The in-farct area decreased significantly after treatment with hypothermia in all the groups, compared with normothermic group( P < 0.05). The longer delayed hypothermia treated with, the larger infact area got. Even 6 hour delayed hypothennia was effective; (3 ) The learning and memory ability in hypothermia group can been improved compared with normothermic group(P < 0.05). Conclusion The protective effect of hypothermia started immediatedly after hypoxic - ischemic is better than delayed hypothennia, 6 hour delayed hypothermia is effective.更多还原