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自交不亲和甘蓝的花粉萌发与花柱内保护酶活性变化

Pollen Germination and Change of Protective Enzyme Activity in Style of Self-Incompatible Brassica oleracea L.

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【作者】 吴能表徐光德唐于婷朱利泉王小佳

【Author】 WU Neng-biao~1,XU Guang-de~1,TANG Yu-ting~1, ZHU Li-quan~2,WANG Xiao-jia~2 1. School of Llife Science, Southwest China Normal University, Chongqing 400715, China; 2. College of Horticulture and Gardens, Southwest China Agricultural University, Chongqing 400716, China

【机构】 西南师范大学生命科学学院西南农业大学园艺园林学院西南农业大学园艺园林学院 重庆 40 0 71 5重庆 40 0 71 5重庆 40 0 71 6重庆 40 0 71 6

【摘要】 研究了柱头提取液对自交不亲和甘蓝花粉萌发、花粉管生长以及自花、异花授粉过程中花柱内过氧化物酶(POD)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)活性动态变化.结果表明:自花柱头提取液能显著抑制花粉萌发和花粉管的生长,而异花柱头提取液能提高花粉萌发率和花粉管的生长;授粉过程中花柱内SOD,POD和CAT活性都显著上升,而自花授粉上升幅度明显低于异花授粉.花柱内蛋白质含量在授粉过程中都会上升,但自花授粉在授粉后最初3~5min出现一个含量峰值.

【Abstract】 Pollen germination, pollen tube growth treated by style extract and activity of SOD, POD, CAT of self-compatible Brassica oleracea L. treated by self-pollination and cross-pollination were discussed. It showed that Pollen germination ratio and pollen tube comparative length were inhibited by self-style extract, but increased by non-self style extract. The activity of SOD, POD and CAT rises up after pollination in styles, self or cross-pollination. But the activity of style treated in cross-pollination rises higher than that in self-pollination. The protein content of styles, after the pollination in both self-pollination and cross-pollination rises at the beginning, then descends after a little while, and the content of protein quickly maintains at higher level. The peak value of content reaches after 3-5 minutes in self-pollination.

【基金】 国家自然科学基金资助项目(39970516;30170646).
  • 【文献出处】 西南师范大学学报(自然科学版) ,Journal of Southwest China Normal University(Natural Science) , 编辑部邮箱 ,2004年05期
  • 【分类号】S635.1
  • 【被引频次】34
  • 【下载频次】372
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