【摘要】 EMS(甲基磺酸乙酯)是烈性致癌物质,对育种者而言,它又是很好的诱变剂。本试验用不同的EMS浓度(0,1 0,1 5,2 0,2 5,3 0mmol/L)和不同时间(12,24,36h)处理甘蓝型油菜的离体小孢子,通过统计每花蕾产胚的数量,观察到随着EMS浓度的增高和处理时间的延长,每花蕾产胚的数量减少,即小孢子的胚胎发生能力减弱。研究得出当EMS浓度为2 5mmol/L,处理时间为24h,每花蕾产胚的数量接近对照产胚数量的一半,根据半致死剂量(LC50)原则,这一浓度和时间对EMS诱变甘蓝型油菜离体小孢子具有重要的应用价值。更多还原

【Abstract】 Although EMS was a strong cancerogen, it was a good mutagen for breeder. The microspores of Brassica napus in vitro were treated by mutagen ethyl methane sulphonate （EMS） at different doses (0,1.0,1.5,2.0,2.5,3.0 mmol·L^-1) and different duration （12, 24, 36 h）.The rerults showed the number of every alabastrum producing embryos decreased,that was the microspore embryogenesis decreased, with the increase of EMS doses and duration of EMS treatment.Every alabastrum producing embryos was about half of the CK when EMS dose was 2.5 mmol·L^-1 and the duration of EMS treatment was 24 h.According to medial lethal concentration(LC₅₀), this EMS dose and duration had important applied value on microspore mutagenesis in vitro in Brassica napus by EMS treatment.更多还原