【摘要】 以滇大蓟成熟叶片为外植体,在添加不同激素的MS培养基中,进行组织培养,建立了滇大蓟再生快繁工厂化生产技术体系。其结果表明:愈伤组织在MS+6 BA2mg/L+NAA0 2mg/L上诱导;愈伤组织接种在MS+6 BA0 5mg/L+NAA0 05mg/L上分化不定芽,继续继代进行芽苗生长并不断增殖;壮苗接于1/3MS基上生根,生根率为99%以上,这样完成植株再生。带根、茎、叶壮苗不必经过炼苗可直接移栽,成活率为89%以上。经研究,不同植物激素浓度配比组合、培养温度对芽苗增殖、生长有影响。更多还原

【Abstract】 Mature leaf blade of Cirsium chlorolepis were used as explant in tissue cultured on MS medium supplemented with different the plant hormones,and the regeneration system in vitro for rapid propagation of Cirsium chlorolepis was established.The results showed:(i)calli were induced in the medium"MS+6-BA 2 mg/L +NAA 0.2 mg/L",(ii)shoots were differentiated and reproduced in the medium "MS+6-BA 0.5 mg/L +NAA 0.05 mg/L",(iii)roots were induced in "1/3 MS"medium and the rooting rate reached 99 %. The regenerated plants were transplanted without training ,the survival rate of transplanting was 89 %.The effects of different combinations of concentrations of the plant hormones,and culturing temperature on multiplication rate of bud and growth vigor of young plant were observed.更多还原