【摘要】 用喜树带芽茎段为外植体,采用正交试验法,研究不同浓度激素对离体愈伤组织和腋芽诱导的影响。试验结果表明:最佳启动培养基和诱导培养基分别为MS+6 BA2 0mg·L-1(单位下同)+IBA1 0和B5+6 BA2 0+IBA0 5。影响离体愈伤组织诱导率的主要因素是BA,影响腋芽萌动率的主要因素为基本培养基。更多还原

【Abstract】 The testing results showed that the best culture medium of initiation and subculture were MS+6-BA2.0+IBA1.0 and B₅+6-BA2.0+IBA0.5. Sections of stems with buds of Camptotheca acuminata Decaisne were used for explant. The effects of plant growth regulators on induction of callus in vitro and axillary bud were investigated by using orthogonal design. The main factor,which affected percentage of callus and germinating rate was 6-BA and the basic medium,respectively.更多还原