【摘要】 目的观察水杨酸钠对肾性高血压大鼠耳蜗Hsf1表达的影响 ,并分析其可能的机制。方法将造模 4周后模型成功大鼠 36只 ,另设正常对照组 (假手术组 ) 12只 ,分为 4组 ,每组 12只 ,A组 :正常对照组 (假手术组 ,Sham组 ) ;B组 :模型对照组 (2K1C组 ) ;C组 :2K1C +水杨酸钠组 ;D组 :2K1C +水杨酸钠 (45 0mg·kg-1·d 1) +L 精氨酸(L Arg ,2 0 0mg·kg-1·d 1)组。采用RT PCR方法检测耳蜗组织Hsf1mRNA表达情况 ,并做半定量法分析。结果① 2K1C +水杨酸钠 +L Arg尾SBP较模型对照组和 2K1C +水杨酸钠组明显降低 (P <0 .0 5 ) ;②正常对照组Hsf1mRNA有微弱表达 ,模型对照组Hsf1mRNA表达较高 ,2K1C +水杨酸钠组表达最高 ,2K1C +水杨酸钠 +L Arg治疗组表达较弱 ;OD值比较分析发现 ,模型对照组Hsf1mRNA表达较正常对照组Hsf1mRNA表达有显著增高(P <0 .0 1) ,2K1C +水杨酸钠组与模型对照组比较有显著性差异 (P <0 .0 5 ) ,2K1C +水杨酸钠 +L Arg治疗组OD值明显低于模型对照组 (P <0 .0 5 )以及 2K1C +水杨酸钠组 (P <0 .0 1) ,2K1C +水杨酸钠 +L Arg治疗组OD值与正常对照组比较无显著性差异 (P >0 .0 5 )。结论水杨酸钠可提高耳蜗组织Hsf1mRNA表达 ,L Arg可使水杨酸钠所致的耳蜗组织Hsf1mRNA表达下调。更多还原

【Abstract】 Objective To study the effects of sodium salicylate on the expression of Hsf1mRNA in cochlear tissues of rats with renovascular hypertension and explore the possible mechanism.Methodes Thirty-six healthy rats were divided into three groups(B:2K1C group;C:2K1C+ sodium salicylate group ;D: L-Arg group) and another 12 rats were added as control group (group A).The animals were sacrificed. After L-Arg was given for four weeks.The expression of Hsf1 mRNA was detected by semi-quantitative RT-PCR in cochlear tissues of rats with renovascular hypertension.The animals were sacrificed after salicylate was given for four weeks.The mRNA expression of Hsf1mRNA was detected by semi-quantitative RT-PCR in cochlear tissues of rats with renovascular hypertension.Results ①SBP of D group was markedly decreased than group B and C.②RT-PCR analysis showed expression of Hsf1mRNA in cochlear tissues of rats with renovascular hypertension.The expression of Hsf1mRNA in group A was weak experssion;while those of group B and C were high;the expression of Hsf1mRNA in group D was remarkablely decreased than those of group B(P<0.05) and C (P<0.01).Conclusion Sodium salicylate could enhance the expression of Hsf1mRNA in cochlear tissues of rats with renovascular hypertension,while the expression of Hsf1mRNA could be decreased by L-Arg.更多还原