【摘要】 以4个甘蓝型黄籽油菜品系为试验对象,采用正交设计,研究了预培养基中2,4-D和6-BA浓度、预培养天数、分化培养基中NAA和6-BA浓度组合、AgNO3浓度等对下胚轴芽再生频率的影响;同时研究了萌发培养基中添加6-BA和NAA对再生频率的影响。结果表明:预培养基中2,4-D及6-BA浓度是影响再生的主要因素;不同材料和不同处理间芽再生频率差异显著,且材料与处理间有明显的互作效应;萌发培养基中添加6-BA和NAA对再生频率无明显影响,但对芽苗的正常形态建成不利。更多还原

【Abstract】 The orthogonal design was used in an experiment, in which the hypocotyls of 4 yellow-seeded rapeseed (Brassica napus L.) lines were cultured and some factors affecting shoot regeneration frequency were investigated. The results showed that concentrations of (2,4-D) and 6-BA in the pre-culture medium were the main factors that affected shoot regeneration. The differences of shoot regeneration frequency among different lines and among different treatments were significant, and line × treatment interaction was noticed. Adding 6-BA and NAA to the germination medium showed no obvious effect on shoot regeneration but it was disadvantageous for normal morphogenesis of the shoots.更多还原