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高免蛋黄抗体中和雏鸭肝炎病毒(DHV)的效价测定
The measurement of neutralizing DHV with hyperimmun yolk antibody
【摘要】 用鸭胚测定半数致死量并进行中和试验.测定了用雏鸭肝炎病毒(Ⅰ型)免疫蛋鸭收获的鸭蛋所制成的蛋黄抗体的滴度.结果表明,雏鸭肝炎病毒(Ⅰ型)的半数致死量为3.50,该毒株的高免蛋黄抗体以1:532稀释可保护50%的鸭胚免于死亡;病毒对照组全部死亡;空白对照组全部存活.该雏鸭病毒性肝炎高免蛋黄抗体滴度较高,可用于预防和治疗雏鸭病毒性肝炎.
【Abstract】 To measure the litration of yolk antibody from duck egg treated by immuning laying ducks with duck hepatitis virus(I). Use duck embryo to measure Lethal dose 50 and do the Neutralization test. Results: Lethal dose 50 of duck hepatitis virus is 3.50, the vaccine strain’s yolk antibodydiluted to 1:532 can protect half of the duck embryo from death; virus contrast group was all dead; blank contrast group all exists. Conclusion: yolk antibody’s antibody titre of the duck virus hepatitis is quite high, which can be used to prevent and treat duck virus hepatitis.
【Key words】 duck hepatitis virus; DHV; duck embryo; yolk antibody; neutralization test;
- 【文献出处】 西南民族大学学报(自然科学版) ,Journal of Southwest University for Nationalities (Natural Science Edition) , 编辑部邮箱 ,2004年05期
- 【分类号】S858.32
- 【被引频次】3
- 【下载频次】279