【摘要】 以禽流感病毒地方株A/chicken/Mudanjiang/ 0 82 3/ 2 0 0 0 (H9N2 )的总RNA为模板 ,用通用反转录引物和自行设计的一对特异性引物 ,采用RT PCR方法扩增了HA基因。测序及序列分析结果表明 :HA基因全长 1 70 7bp ,编码 560个氨基酸残基 ,与GenBank收录的H9N2亚型的核苷酸同源性最高达 99% ,最低为 87 2 3 % ;氨基酸同源性最大达 98 0 4 % ,最低为 88 57%。经HA裂解位点附近的氨基酸序列分析表明 ,CMJ/ 0 0毒株为低致病力毒株更多还原

【Abstract】 The total RNA extracted from local isolated H9N2 avian influenza virus (A/chicken/Mu dan jiang/0823/2000) was used as the template in this study, a piece of universal primer and a pair of specific primer were designed for RT-PCR to amplify the HA gene. A specific 1707 bp cDNA product was obtained, which encodes 560 amino acid residues, and the uncleotide homology with H9N2 subtype publicized in Genbank was between 99% and 87.23%. Furthermore, the homology was between 98.04% and 88.57% at amino acid level, and the amino acid sequence of HA cleavage site indicated that CMJ/00 strain had low virulence.更多还原