节点文献
类固醇激素对牛输卵管上皮细胞特异性糖蛋白基因表达作用的研究
Effects of Different Steroid Hormones on Expression of Bovine Oviductal Epithelial Specific Glycoprotein Genes in Cultural Bovine Oviductal Epithelial Cells
【摘要】 模拟发情期母牛体内类固醇激素水平,在培养乏情期母牛输卵管上皮细胞的体系中添加雌二醇(E2)、孕酮(P)和血小板激活因子(plateletactivatingfactor,PAF),以诱导细胞的分泌作用。借助Northern的斑点杂交技术分析输卵管上皮特异性糖蛋白(OGP)基因的mRNA水平,研究激素及生长因子对体外培养的乏情期母牛输卵管上皮细胞分泌OGP的影响。结果显示:E2、P和PAF可以促进乏情期母牛输卵管上皮细胞OGP基因的表达。
【Abstract】 We have evaluated effects of 17β-estradiol (E2), progesterone (P), platelet activating factor (PAF), E2and PAF, P and PAF on expression of bovine oviductal epithelial OGP genes in cultural bovine ampullary oviductal epithelial cells from nonestrus with Northern dot-blotting. The result of dot-blotting showed that OGP mRNA existed in the cultural oviductal epithelial cells induced by E2, P and PAF.
【关键词】 输卵管上皮特异性糖蛋白;
雌二醇;
孕酮;
血小板激活因子;
mRNA;
【Key words】 specific oviductal epithelial glycoprotein; 17β-estrodial; progesterone; platelet activating factor; mRNA;
【Key words】 specific oviductal epithelial glycoprotein; 17β-estrodial; progesterone; platelet activating factor; mRNA;
【基金】 中国农业科学院院长基金(9725-16)
- 【文献出处】 畜牧兽医学报 ,Chinese Journal of Animal and Veterinary Sciences , 编辑部邮箱 ,2004年06期
- 【分类号】S852.2
- 【被引频次】6
- 【下载频次】85