【摘要】 从带有鹅细小病毒(GPV)NS1基因的重组质粒pMD18T NS1用限制性内切酶EcoRI和BamHI双酶切回收1880bp大小片段,并制备出地高辛标记的GPV核酸探针。其标记效率达到0 01pg/μl。特异性检测结果表明,该探针能与GPV不同毒株核酸发生特异性杂交,而与对照的DPV、GPPV等病毒的核酸杂交反应均为阴性;敏感性检测结果表明,该探针对GPV的最低检出量为0 0224pg。该探针对不同方法处理的GPV感染病料进行检测,均出现杂交阳性。表明所研制的标记探针用于GPV的检测是可行的。更多还原

【Abstract】 The Goose parvovirus (GPV) 1880 bp NS1 DNA fragment was recuperated from its recombinant plasmid and labeled with Digoxigenin prepared DNA probe for detection of GPV.The hybridization assay result of specificity showed all the DNA of GPV strains were positive,but other nucleotide extracted from DPV,GPPV and allantoic fluid of the nuinoculated embryonating eggs were negertive.The hybridization assay result of sensitivity showed as little as 0.0224 ng of GPV DNA could be detected by DIG-labeled probe.This probe could also hybridizate with GPV neucleotide extracted from the clinical samples which were treated different ways.So this DIG-labeled DNA probe could be used to detect the GPV.更多还原