【摘要】 目的 :探讨简易的成人成骨细胞体外培养方法 ,为进一步研究成骨细胞及骨组织工程提供技术平台。方法:手术中抽取健康成人骨髓组织 ,采用全骨髓培养法在体外进行培养 ,细胞汇合后改用含地塞米松、β-甘油磷酸钠和维生素 C的条件培养基进行传代培养 ,观察细胞增殖和分化情况 ,并对细胞进行鉴定。结果:培养的成人成骨细胞表现出成骨细胞的形态特征 ,细胞碱性磷酸酶 (AL P)阳性率为 6 8% ,经 Von Kossa’s染色证实其能最终形成钙结节。结论:全骨髓培养法所培养的细胞具有成骨细胞的特性 ,可作为骨组织工程种子细胞培养的常规方法。更多还原

【Abstract】 Objective: To create the model of human bone marrow stromal cell (BMSc) separation and culture in vitro. Methods: Adult bone marrow about 3～5 ml were drawed by puncture,then cultured in DMEM containing volumes of 15% fetal bovine serum. After they grew on a great mess of surface,the adhesive cells were preserved for culture. BMSc were cultured in a conditional medium in subculture including dexamethasone,vitamin C,and β-glycerophosphate. Proliferations and differentiations of all the cultured cells were observed and identificated. Results: The adult human bone marrow stromal cell cultured in this experiment demonstrated a biological and morphologic characteristics of osteoblasts. Alkaline phosphatase (ALP) activity of BMSc at passage 2 were 68%.The mineral nodules were observed by Von Kossa’s stain. Conclusions: The cells cultured use the method this experiment have characteristic of osteoblasts.This method can be used as a roution method in bone tissue engineering.更多还原