【摘要】 用微玻璃珠研磨法将 p ARG7质粒转入到莱茵衣藻精氨酸依赖型、缺壁突变株品系 CC-1 61 8( arg7cw1 5 mt-)和 CC-1 61 7( arg7cw1 5 mt-)中 ,得到了一些不同的插入型突变转化子 .CC-1 61 7突变株品系的转化率为 72个 /μg DNA,CC-1 61 8突变株品系的转化率为 2 96个 /μg DNA,即转化率比 CC-1 61 7突变株品系高 .经多次转化共获得各种转化子 3 90 0多个 ,对它们进行荧光检测后检测出缺乏叶绿素 b的两个突变子ARGT3和 ARGT4,并进一步用高效液相色谱法对其光合色素含量进行检测结果证实 ,这两个突变体的确丧失了叶绿素 b合成能力 .它们将可作为 CBN1基因的克隆及其定位的有用的工具更多还原

【Abstract】 The plasmid p389ARG7 is transformed into arginine dependent and cell wall-less strains CC-1618 (arg7cw15mt-) and CC-1617 (arg7cw15mt-) of Chlamydomonas reinhardtii by using glass bead method respectively, and some insertion mutated transformants with various background have been obtained. The transformation rate of the CC-1617 is 72 per μl DNA while that of the CC-1618 is 296 per μl DNA ,which shows that the transformation efficiency of latter is higher than that of the former. At last above 3 900 transformants have been obtained and fluorescence test is performed to screen them, from that 2 chlorphyll b-less mutants ARGT3 and ARGT4 have been detected and isolated . The further result of detection of photochrome contents screened by HPLC method demonstrates that each of the 2 mutants had lost the ability of biosynthesis chlorophyll b, and therefore they will become useful tool for the cloning and localization of the CBN1 gene.更多还原