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反相液相色谱法测定血清中L-色氨酸及其代谢产物
Determination of L-tryptophan and Its Metabolites by RP-HPLC
【摘要】 建立了反相高效液相色谱-紫外检测法同时测定血清中L-色氨酸、5-羟色胺和5-羟吲哚乙酸的方法。血清中L-色氨酸、5-羟色胺和5-羟吲哚乙酸的添加回收率分别为92.1%,90.8%和71.1%。该法快速准确、灵敏度高,且重现性好。
【Abstract】 A rapid and specific method for the determination of L-tryptophan, serotonin and 5-hydroxyindoleacetic acidin pig,s serum has been established by high performance liquid chromatography with UV detector. After standard solutionadded, the good recovery of L-tryptophan, serotonin and 5-hydroxyindoleacetic acid were 92.1%, 90.8% and 71.1% respectively.
【关键词】 反相高效液相色谱法;
L-色氨酸;
5-羟色胺;
5-羟吲哚乙酸;
血清;
【Key words】 RP-high performance liquid chromatography; L-tryptophan; serotonin; 5-hydroxyindoleacetic acid; serum;
【Key words】 RP-high performance liquid chromatography; L-tryptophan; serotonin; 5-hydroxyindoleacetic acid; serum;
【基金】 国家自然科学基金项目:创新研究群体科学基金,项目编号:30121004。
- 【文献出处】 现代科学仪器 ,Modern Scientific Instruments , 编辑部邮箱 ,2004年04期
- 【分类号】O657.72
- 【被引频次】11
- 【下载频次】327