【摘要】 葡萄糖感应型智能化胰岛素释放系统的研究正受到越来越广泛的重视和关注。本文系统研究了具有等离子体接枝聚丙烯酸高分子链和固定葡萄糖氧化酶的开关膜的响应特性,接枝在多孔聚偏氟乙烯膜孔上的聚丙烯酸链起到pH感应开关的作用,固定的葡萄糖氧化酶起到葡萄糖传感器和将葡萄糖转化为葡萄糖酸的催化剂作用。研究结果表明,该开关膜对葡萄糖的感应性极大程度地依赖于聚丙烯酸的接枝率,因为膜孔的pH响应性决定了其葡萄糖感应控释特性。因此,为了获得良好的葡萄糖感应开关特性,聚丙烯酸的接枝率必须控制在一个适当的范围。对于聚丙烯酸接枝率为1.55%的开关膜,当外界葡萄糖浓度从0上升到0.2 mol/L时,胰岛素的扩散透过系数猛然增大了9.37倍,显示出了良好的葡萄糖感应型自调节式胰岛素释放特性。更多还原

【Abstract】 The pore size and permeability control of a glucose-responsive gating membrane with plasma-grafted poly(acrylic acid)(PAAC) gates and covalently bound glucose oxidase(GOD) enzymes were investigated systematically.The PAAC-grafted porous polyvinylidene fluoride(PVDF) membranes with a wide range of grafting yields were prepared using a plasma-graft pore-filling polymerization method,and the immobilization of GOD was carried out by a carbodiimide method.The linear grafted PAAC chains in the membrane pores acted as the pH-responsive gates or actuators.The immobilized GOD acted as the glucose sensor and catalyzer;it was sensitive to glucose and catalyzed the glucose conversion to gluconic acid.The experimental results showed that,the glucose-responsivity of the solute diffusional permeability through the proposed membranes was heavily dependent on the PAAC grafting yield,because the pH-responsive change of pore size governed the glucose-responsive diffusional permeability.It is very important to design a proper grafting yield for obtaining an ideal gating response.For the proposed gating membrane with a PAAC grafting yield of 1.55%,the insulin permeation coefficient after the glucose addition(0.2 mol/L) was about 9.37 times that in the absence of glucose,presenting an exciting result on glucose-sensitive self-regulated insulin permeation.更多还原