【摘要】 目的　建立反相高效液相色谱法测定丙酸氯倍他索透剂含量的方法。方法　以shim PackODS为色谱柱 ,以 0 .0 5mol/L磷酸二氢钾溶液 (用 85 %磷酸调节 pH为 2 .6± 0 .0 1 ) 乙腈 (30∶70 )为流动相 ,检测波长为 2 4 0nm ,流速为 0 .8mL/min。结果　丙酸氯倍他索浓度在 2 0 .9～ 1 2 5 .6 μg /mL之间与峰面积线性关系良好 ,r =0 .9998,n =5 ;回收率为 1 0 0 .6 8%,RSD =0 .5 2 %,n =6。结论　本法准确、快速、简便、重现性好 ,且不受辅料干扰 ,适用于该制剂的含量测定。更多还原

【Abstract】 OBJECTIVE The method for determination of clobetasol propionate liniment by RP-HPLC was established. METHOD A Shim-Pack ODS column was adopted. The mobile phase consisted of 0.05mol/L potassium dihydrogen phosphate ( adjusted to pH 2.6±0.01 by 85% phosphoric acid ) -acetonitrile (30∶70) with the flow rate of 0.8mL/min. UV detective wavelength was 240 nm.RESULTS Linear range of clobetasol propionate was 20.9～125.6μg /mL with r=0.9998 (n=5). The recovery was 100.7% and RSD=0.52% (n=6).CONCLUSION The method is accurate,reliable, simple and reproducible, it can be used for quality control of this preparation.更多还原