【摘要】 目的　本实验通过定量和定性两部分为通脉丸建立了质量标准。方法　采用TLC法对丹参、川芎和鸡血藤分别进行定性鉴别。采用超声波提取丹参酮ⅡA ,应用高效液相色谱法测定丹参酮ⅡA含量 ,色谱柱为KromasilC18柱 (2 5 0mm× 4 .6mm ,5 μm) ,流速为 1.2mL/min ,流动相为甲醇 水 (80∶2 0 ) ,检测波长为 2 6 8nm。 结果　在TLC色谱中均能检出丹参、川芎、鸡血藤。丹参酮ⅡA的线性范围在 0 .16～ 0 .4 8g(r =0 .99998,n =5 ) ,平均回收率为 10 0 .0 5 % (RSD =2 .98% ,n =5 )。 结论　此定量定性方法准确可行 ,重复性好 ,可作为通脉丸的质量控制标准更多还原

【Abstract】 OBJECTIVE To establish the quality standard for Tongmai Pills through quantitative and qualitative experiments. METHOD Radix Salvia,Rhizoma Chuanxiong and Caulis Spatholobi were identified by TLC. Tanshinone ⅡA was extracted by ultrasonic method and determined by HPLC .Column was KromasilC 18 column(250mm×4.6mm,5μm),the mobile phase with a flow rate of 1.2mL/min was methane-water (80∶20),the detection wavelength was 268nm.RESULTS Radix Salvia,Rhizoma Chuanxiong and Caulis Spatholobi could be identified by TLC. Determination method of tanshione ⅡA was linear at range of 0.16～0.48μg ( r=0.99998, n=5) , the average recovery was 100.05% ( RSD=2.98% n=5 ). CONCLUSION The quantitative and qualitative analysis methods are accurate and reproducible. This study provides a method for the quality control of tongmai pills.更多还原