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紫外分光光度法测定复方红霉素洗剂中甲硝唑的含量
Determination of metronidazole in the compound erythromycin lotion with UV-spectrophotometry
【摘要】 目的 建立复方红霉素洗剂中甲硝唑的含量测定方法。方法 采用紫外分光光度法 ,以盐酸溶液 (9→ 10 0 0 )为溶剂 ,测定波长为 2 77nm。结果 甲硝唑的检测线性范围为 3~ 2 1μg·mL-1,r=0 .9999,平均回收率为 99.83%。结论 本法操作简便 ,准确性好 ,可作为复方红霉素洗剂中甲硝唑的含量测定方法。
【Abstract】 OBJECTIVE To establish an UV-spectrophotometry to determine the content of metronidazole in the compound erythromycin lotion. METHOD The hydrochloric acid solution (9→1000) was used as solvent. The wavelength of detection was 277nm.RESULTS The calibration curve was linear(r =0.9999) within the range of 3~21μg·mL -1 of metronidazole.The average recovery was 99.83%.CONCLUSION The method is simple and accurate, and can be applied to determine the metronidazole content in the compound erythromycin lotion.
【关键词】 紫外分光光度法;
复方红霉素洗剂;
甲硝唑;
含量测定;
【Key words】 UV-spectrophotometry; compound erythromycin lotion; metronidazole; determination;
【Key words】 UV-spectrophotometry; compound erythromycin lotion; metronidazole; determination;
【基金】 深圳市福田区卫生科技资助项目,编号 :FT2 0 0 2 2 9
- 【文献出处】 中国现代应用药学 ,The Chinese Journal of Modern Applied Pharmacy , 编辑部邮箱 ,2004年02期
- 【分类号】R927.2
- 【下载频次】58