【摘要】 目的　建立测定人血浆中头孢泊肟的反相高效液相色谱检测法。方法　血浆样品用高氯酸溶液沉淀后上清液直接进样。色谱柱为C18柱 ,15 0mm× 4 .6mm ,5 μm ,流动相为 1.5 0mmol·L-1高氯酸溶液 乙腈 (85∶15 ) ,流速为 1.2 0mL·min-1,紫外检测波长 :2 5 4nm。外标法峰面积定量。结果　测定方法在 0 .10～ 6 .0 0mg·L-1内具良好的线性关系 ,回收率为 98.0 %～ 110 .0 % ,日内、日间RSD小于 13% ,最低检测浓度为 0 .10mg·L-1。结论　本测定方法具灵敏、准确、快速的优点 ,适用于头孢泊肟酯片剂的药代动力学和生物利用度研究。更多还原

【Abstract】 OBJECTIVE To develop a simple, sensitive and rapid RP-HPLC method for the determination of cefpodoxime in human plasma. METHOD The plasma samples were extracted with 1.25 mol×L -1 perchloric acid. The analysis involved a 150mm×4.6mm ID column packed with C 18( 5μm ). The mobile phase consisted of 1.50 mmol·L -1 perchloride acid solution-acetonitrile( 85∶15 ) with a flow rate of 1.20 mL·min -1. The UV detector were set at 254nm.RESULTS A good linearity was obtained in the rang of 0.10 ～ 6.00 mg·L -1( r=0.9999 ). The recovery was between 98.0% ～ 110.0%. The relative standard deviation of within-day and between-day were less than 13% ( n=6 ). The minimal detectable concentration in plasma was 0.10 mg·L -1. CONCLUSION The established method is sensitive, accurate and simple for the determination of cefpodoxime levels in human plasma. It is suitable for the pharmacokinetics and bioavailability study of cefpodoxime proxetil.更多还原