【摘要】 目的观察早期生长反应基因(earlygrowthresponsegene-1,Egr-1)在直肠癌组织的分布规律,探索Egr-1在直肠癌治疗中的作用及意义。方法采用随机、单盲、对照研究。将癌组织组85例直肠癌及对照组13例直肠腺瘤切除病例标本的病理切片分为癌中心组织,癌周上、下缘组织,癌旁(距癌缘0.5cm)上下缘组织,远近切缘组织及瘤中心组织,瘤旁组织,远近切缘组织进行Egr-1免疫组化测定。结果癌组织组85例癌组织与正常组织(经病理检查证实的正常切缘组织)比较,差异有非常显著性意义(F=14.5,P<0.01);远端癌旁组织与近端癌旁组织比较,差异有显著性意义(F=7.28,P<0.05);获随访5年的72例中出现局部复发20例,与未发现局部复发的52例癌旁组织比较,差异有显著性意义(F=19.3,P<0.05)。对照组直肠腺瘤组织与其切缘正常组织比较,差异有显著性意义(F=4.3,P>0.05)。结论Egr-1在直肠癌的发生或/和发展中具有明确的抑癌作用,且可作为预测术后局部复发的独立指标,为低位直肠癌保肛手术治疗提供了“基因层面”的理论依据。更多还原

【Abstract】 AIM:To observe the distributions of early growth response gene 1 (Egr 1) in rectal cancer tissues and to explore the role and significance of Egr 1 in the treatment of rectal cancer.METHODS:A randomized, controlled and single blind trail was conducted. The pathological sections of 85 specimens with rectal cancer in cancer tissue group and 13 specimens with rectal adenomas in control group were divided into center tissues of cancer, upper and below edge tissues around cancer, paracarcinoma tissues(0.5 cm from the border), upper and below edge normal tissues, distant incision edge tissues and proxinmal incision edge tissues, center tissues of tumors, Egr 1 immuohistochemical assay was performed for estimation of distal incision edge tissues and proximal incision edge tissues of tumors and tissues around cancer.RESULTS:Significant differences were found when totally 85 specimens of cancer tissues were compared with normal tissues(the normal incisal edge tissue tested and confirmed by pathological test)(F=14.5,P< 0.01), the distal paracarcinoma tissues was compared with proximal paracarci noma tissues (F=7.28,P< 0.05). Twenty of the 72 follow up were found local recurrence within 5 years. There was a significant difference compared with 52 cases of proximal cancer tissues that had no local recurrence(F=19.3,P< 0.05).There was no significant difference between the rectal gland tissues in the control group and the normal tissues of incisal edge(F=4.3,P >0.05). CONCLUSION:Egr 1 has the anti cancer effect on the occurrence and development of rectal cancer, which can be taken as an independent market for preventing local occurrence after operation and supply theoretical basis of “gene”for the anus preserving operation for low rectal theoretical cancer.更多还原