【摘要】 目的:观察复方鳖甲软肝方药物血清对离体肝星形细胞(hepaticstellatecells,HSC)转型的影响,以探讨该药物血清防治肝纤维化作用的机制。方法:将成年的健康雄性SD大鼠75只随机分为对照组、模型组、高、中、低剂量药物组共5组,并制备各组血清。采用大鼠HSC分离技术,将分离得到的HSC分别添加各组血清培养24,48h后,免疫组化ABC法检测各组HSC的α-平滑肌肌动蛋白(α-smoothmuscleactin,α-SMA)表达,经图像分析系统定量分析。结果:培养24,48h各药物血清组血清对HSC中α-SMA阳性染色不同时相吸光度(A值)(24h:高、中、低剂量分别为110.95±45.21,243.82±62.97,417.46±126.72;48h分别为:333.71±57.90,345.08±51.85,675.35±202.20)与模型组(24h:778.93±190.26;48h:1032.69±106.64)比较,差异有显著性意义(P<0.05);模型组、高、中剂量药物血清组与正常组比,差异有显著性意义(P<0.05)。结论:高、中剂量复方鳖甲软肝方药物血清能抑制HSC的α-SMA表达,其抗肝纤维化作用机制可能与药物能抑制HSC向α-SMA的转型有关。更多还原

【Abstract】 AIM:To observe the influence on conversion of myofibroblasts(MFB) from hepatic stellate cells(HSC) in vitro by drug serum of fufang biejia ruangan fang,and to explore its mechanism in preventing the hepatic fibrosis. METHODS:Seventy five adult healthy male SD rats were randomly divided into normal control group, model group,high,middle and low dose groups(fufang biejia ruangan fang),and the serum was prepared in all the groups.The HSCs were separated with the HSC separating technique,and then cultured in different serum for 24 and 48 hours,then the expression of alpha smooth muscle actin(α SMA) in HSC was detected with immunohistochemistry ABC,and then the quantitative analysis was performed by image analysis system. RESULTS:The absorbance(A) of the serum to the different α SMA positive staining in HSC in the high,middle and low dose drug groups at 24 hours(110.95±45.21,243.82±62.97 and 417.46±126.72) and 48 hours(333.71±57.90,345.08±51.85 and 675.35±202.20) were significantly different from those in the model group[778.93±190.26 and 1 032.69±106.64](P< 0.05);those in the model group, high and middle dose drug group were markedly different from that in the normal control group(P< 0.05).CONCLUSION:Drug serum of highand middledose fufang biejia ruangan fang can inhibit the expression of αSMA in HSC.And its mechanism of curing hepatic fibrosis may be related with the inhibition of the conversion of αSMA from HSC by drug serum of fufang biejia ruangan fang.更多还原