【摘要】 目的:探讨新型的人工合成的可降解材料Poly(DL-lactide-co-ε-caprolac-tone)修复周围神经损伤的可行性。方法:将Poly(DL-lactide-co-ε-caprolactone)用特殊方法制成长10mm,内径1.3mm,壁厚0.5mm的神经套管。健康成年SD大鼠24只,随机分为3组,每组8只。实验组用Poly(DL-lactide-co-ε-caprolactone)神经套管,对照I组用自体神经移植,对照II组用硅胶管桥接。3组均修复5mm长坐骨神经缺损,术后3个月分别观察3组大鼠的足跟溃疡时间、步态、关节活动,计算坐骨神经指数(sciaticnervefunctionindex,SFI),测量坐骨神经动作电位的潜伏期、波幅和传导速度。并行常规苏木精-伊红(HE)染色和S-100免疫组织化学染色(常规ABC法),以及运动终板的免疫组织化学和透射电镜观察超微结构。结果:实验组术后3个月各组坐骨神经动作电位的波幅和传导速度与对照II组比较,差异有显著性意义;HE染色显示神经套管桥接处神经结构连续性良好,移植段可见神经纤维通过,同时可见新生的血管。S-100免疫反应阳性的雪旺氏细胞沿神经纤维分布成条带状。运动终板乙酰胆碱酯酶阳性反应加深,于腓肠肌的中上部形成终板带,经结合镀银染色可见再生神经束及发出的分支与运动终板相连。电镜观察超微结构接近正常的运动终板。结论:Poly(DL-lactide-co-ε-ca更多还原

【Abstract】 AIM:To explore the feasibility of using the novel biomaterials,Poly(DL lactide co ε caprolactone), to repair the peripheral nerve defect. METHODS:A novel technique was used to create porous conduits with the length of 10 mm, the inside diameter of 1.3 mm and the wall thickness of 0.5 mm. Twenty four healthy SD rats were randomly divided into 3 groups, with 8 rats in each group.Poly(DL lactide co ε caprolactone)conduits were used in experimental group, autografts in control group Ⅰ and silicon conduits in control group Ⅱ for repairing 5 mm sciatic neurologic defect. Foot ulceration, gait, joint movement, and sciatic nerve function index(SFI) were observed. The latency, amplitude and conduction velocity of action potential were measured.Hematoxyline eosin(HE)staining and S 100 immunohistochemistry(routine ABC method) were used, and the ultrastructure was observed immunohistochemically under transmission electron microscope(TEM). RESULTS: Three months after operation, the amplitude and conduction velocity of the action potential of sciatic nerve in the experimental group were significantly different from that in control group Ⅱ. HE staining showed that there were newborn blood vessels and continuous regenerated myelinated nerve fibers under light microscopy.Schwann cells with positive S 100 immunoreaction showed a strip shaped distribution along the regenerated nerve fibers.The positive reaction of acetylcholinesterase(AchE) on motor end plate was deepened. End plate was formed in the middle and superior part of gastrocnemius. The regenerated fasciculus and the branchs were connected with motor end plate through argentation staining.Microstructure of motor end plate was nearly normal under TEM. CONCLUSION:Poly(DL lactide co ε caprolactone) conduit is useful in the repair of perpheral nerve defect. The possibility of designing artificial nerve by Poly(DL lactide co ε caprolactone)can be investigated further.更多还原