【摘要】 胃泌素通过胃泌素受体介导,刺激胃酸的分泌,又维持胃黏膜的完整性。胃泌素与脾虚的关系密切,脾虚病理过程中胃泌素水平处于紊乱状态。本研究观察到:脾虚大鼠胃壁细胞胃泌素受体结合位点数下降而受体亲和力升高;与胃黏膜保护机制相关的一氧化氮在胃黏膜中含量下降;经五肽胃泌素腹腔注射刺激后的胃酸分泌反应性显著升高。经益气健脾复方补中益气汤治疗后,以上紊乱状态均获得一定程度的恢复。另证明体外补中益气汤含药血清给药能与胃泌素受体竞争性结合。总之,文章阐明了补中益气汤的健脾机制与调节胃泌素相关。更多还原

【Abstract】 Buzhong Yiqi decoction(BYD) is a classic recipe for replenishing qi and invigo rating spleen.Gastrin stimulates the secretion of the gastric acid,promotes the production of the cells in gastric mucosa membrane,and maintains the integrality of the mucous membrane.It has been proved that gastrin is closely related to sp leen-deficiency,and the content of gastrin is in disorder during the course of spleen-deficiency.Our laboratory has already successfully established the metho d of isolating the gastric parietal cells and measuring the gastrin receptor bin ding capacity in rats,and found the binding capacity of gastrin receptor in isol ated gastric parietal cells more markedly decreased in spleen-deficiency rats t han in the normal rats.After treated with huangqi,a kind of herb attributed to i nvigorating spleen and replenishing qi,huangqi,the decreased binding capacity is improved.In the study the subjects observed further are: the changes of the bin ding capacity and affinity of the gastrin receptor in isolated gastric parietal cells in spleen-deficiency rats;the effect of the serum containing BYD on gastr in receptor in isolated gastric parietal cells in vivo in rats;the influence of BYD on the amount of nitric oxide(NO) in gastric mucosa in spleen-deficiency ra ts;whether BYD can regulate the sensitivity of the gastric acid secretion stimul ated by gastrin through celiac injection in spleen-deficiency rats,and with whi ch the changes of gastrin receptor are associated.In all,the study is about the effect of BYD on gastrin receptor in gastric parietal cells and its associated i nvigorating spleen mechanisms in spleen-deficiency rats.Meanwhile the essence o f the syndrome of spleen deficiency from the level of gastrin receptor and molec ular signal transduction in the isolated parietal cells is also explored.更多还原