【摘要】 以PC12细胞为实验材料,研究低频脉冲电场(f=50 Hz,τ=20 ms,Epp=1 V／m)对神经细胞突起生长及膜受体聚簇的影响。结果显示,该电场能促进NGF受体的聚簇。电场处理15 min使PC12细胞表面的NGF受体发生明显的聚簇,30 min组次之,5 min组聚簇效果较弱。这表明细胞膜受体可能是电磁场与细胞相互作用的位点之一。运用细胞突起图形处理软件,追踪测定经电场处理后PC12细胞的突起数量和长度,发现该电场能显著促进细胞突起的生长,但对突起数量没有明显的影响。更多还原

【Abstract】 PC12 cells were exposed to low-frequency pulsed electrical field (f=50 Hz, τ=20 ms, Epp=1 V/ m) to study the possible effects of electrical field exposure on nerve growth factor receptor (NGFR) and outgrowth in PC 12 cells. The results showed that pulsed electrical field could obviously induce the clustering of NGFR. The clustering effect was strongest after exposure to low-frequency pulsed electrical field for 15 min, just as the effect of NGF. But it was weakest after exposure for 5 min. It suggested that receptor in the cytoplasmic membrane would be the site where low-frequency pulsed electrical field interacted with cells. By an image-analysis software, it was showed that low-frequency pulsed electrical field promoted outgrowth of PC 12 cells.更多还原