【摘要】 以 4 8 2× 5 0 0 3的 1 6 6个F2 单株为作图群体 ,利用 1 35个RFLP探针和 1 31对SSR引物对亲本 4 8 2、5 0 0 3之间的多态性进行了检测 ,筛选出 1 0 9个RFLP多态性探针和 81对SSR多态性引物用于F2 群体分析 ,利用上述 1 0 9个RFLP标记和 81个SSR标记 ,构建了具 1 90个RFLP、SSR标记 1 99个标记位点的玉米分子遗传图谱 ,覆盖整个基因组 2 984 1cM ,标记间平均间距 1 5 1cM。更多还原

【Abstract】 F 2 individuals derived from the single cross of inbred lines 48 2 and 5003 were used as mapping population in maize ( Zea mays L.).135 genomic or cDNA RFLP probes and 131 SSR primer pairs were used to detect the RFLP and the SSR polymorphisms between parents 48 2 and 5003.109 RFLPs and 81 SSR primer pairs indicating the polymorphisms were used in analyzing F 2 population.Using the same 109 RFLP markers and 81 SSR primer pairs,a molecular genetic linkage map was constructed on which 199 loci of 190 markers distribute among 10 linkage groups and span maize genome about 2984.1 cM with average distance of 15.1 cM between markers.更多还原