【摘要】 以甘肃漳县当年的当归(Angelicasinensis(Oliv.)Diels)种子经萌发而得的幼嫩水培苗为材料进行组织培养研究,结果表明:最佳诱导愈伤组织的培养基是H+0 5mg/L2,4-D;产生愈伤组织的能力依次是叶柄,根尖,叶片;愈伤组织的最佳增殖培养基是MS+1 0mg/L2,4-D+0 2mg/L6-BA;水培苗的最佳壮苗、增殖培养基是MS+1 0mg/LIAA+300mg/LLH+5 0mg/L6-BA;最佳生根培养基是MS+1 0mg/LIAA+300mg/LLH.更多还原

【Abstract】 The tissure culture of the plantlet germinated from seed of Angelica sinensis(Oliv) Diels in Zhangxian county,Gansu is studied.The results show that: 1.H+2,4-D 0.5 mg/L is beneficial to induce callus;2.The callus inducation from the petioles has arisen rapidly and increased at a high rate;3.Better proliferation medium of callus is MS+2,4-D 1.0 mg/L +6-BA 0.2 mg/L;4.Better strong shoot and proliferation medium is MS+IAA 1.0 mg/L +6-BA 5 mg/L+LH 300 mg/L;5.Inducation of root is better in medium of MS+IAA 1.0 mg/L +LH 300 mg/L.更多还原