4 kinds of predominant G~+ bacteria isolated from mastitis bovine milk were used to prepare lysate vaccine mixed with 3 sorts of adjuvants.After immunization,the rabbits blood samples were collected every 7 d from 7th day postimmunization.In order to determine if these 4 kinds of predominant G~+ bacteria have better antigenicity and select proper adjuvant,we collected sera to determine agglutination titer by agglutination reaction test tube.The result revealed,agglutination titer has arised after immunizati...