【摘要】 从伤诱导的黄河蜜甜瓜(Cucumismelocv.Huanghemi)成熟果实的中果皮组织中分离总RNA,经反转录和PCR扩增得到974bp的cDNA片段,克隆到质粒载体pGEM-3zf,经测序与Genbank中甜瓜1-氨基环丙烷1-羧酸合成酶(1-amino-cyclopropane-1-carboxylieacidsynthase,ACS)基因同源性为99%。克隆片段经双酶切消化,反向插入到植物表达载体pBI121的35S启动子和NOS终止子之间,构建成ACS基因的反义表达载体。更多还原

【Abstract】 A cDNA fragment of an amino-cyclopropane-1-carboxylie acid (ACC) synthase was amplified by Reverse Transcription Polymerase Chain Reaction (RT-PCR) from wounded mesocarp tissue of mature muskmelon (Cucumis melo cv.Huanghemi) and cloned into a vector pGEM-3zf.DNA sequencing showed that the cloned fragment shared highly homogeneity to the cDNAs of ACC synthases from other Cucubitae plants.The cloned cDNA of ACC synthase was further introduced into a binary vector pBI121 in a reverse orientation with its upstream promotor (CaMV 35S),giving an expressing plasmid containing the antisense ACC synthase gene.更多还原