【摘要】 用植物源病毒抑制物WCT- 直接处理烟草花叶病毒(TMV)病毒粒体,经枯斑半叶法接种和电镜检测发现,处理后的病毒致病力明显下降,病毒粒体大量发生断裂、略变弯曲等畸变现象,WCT- 对TMV的枯斑抑制率为86.6%;WCT- 处理烟草后,其叶片匀浆和胞间液的蛋白经SDS-PAGE不连续电泳发现,处理后的烟草叶片胞间在第5天至第9天和第7天分别存在1条酸溶性蛋白和1条碱溶性蛋白。上述研究结果表明,WCT- 不仅对TMV病毒粒体具有体外钝化作用,而且能诱导植物产生病程相关蛋白,提高烟草抵抗TMV侵染的能力。更多还原

【Abstract】 After treated by Antiviral agent WCT-Ⅱ from plants, virus pathogenicity was decreased obviously by withered spot half leaf′s method and virus microsomes were broken up in large number by electron microscopy.Spraying WCT-Ⅱ on leaf of tobacco induced,the proteins existed in leaves and intercellular was analyzed through SDS-PAGE , the result showed that a specific acid-soluble protein during the 5th-9th day and a specific basic-soluble protein in the 7th day exist in tobacco′s intercellular . The research suggested that the virucide agents not only can destroy virus, but also enhance plant′s resistance against invading pathogens.更多还原