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毛豹皮樟的叶片DNA提取及其RAPD引物筛选
DNA Extraction and RAPD Primer Screening in Leaves of Litsea coreana var. lanuginosa
【摘要】 以毛豹皮樟叶片为材料,对其DNA提取方法和RAPD引物的筛选进行了研究。结果表明,采用改良SDS法从毛豹皮樟的老、幼叶片中都能提取到高质量的DNA,可以用于RAPD分析;从4组随机引物中筛选出了16个显示毛豹皮樟遗传差异的多态性引物。
【Abstract】 The method of isolating genomic DNA and RAPD primer screening of were studied. The results are as follows; Qualified DNA could be obtained from Litsea coreana var. lanuginose by means of the improved SDS method. The DNAs are qualified for RAPD analysis. At.the same time, 16 arbitrary 10 - mer primers were selected from 4 groups to show much higher genetic diversity of L. coreana var. lanuginose.
【关键词】 毛豹皮樟;
叶片;
DNA提取;
RAPD引物筛选;
【Key words】 Litsea coreana var. lanuginose; leaf; DNA isolating; RAPD primer screening;
【Key words】 Litsea coreana var. lanuginose; leaf; DNA isolating; RAPD primer screening;
- 【文献出处】 西北林学院学报 ,Journal of Northwest Forestry University , 编辑部邮箱 ,2004年04期
- 【分类号】S792
- 【被引频次】12
- 【下载频次】181