【摘要】 目的 :在大肠杆菌中表达SARS病毒S2与硫氧还原蛋白 (Trx)的融合蛋白 ,并对其进行血清学鉴定。方法 :克隆编码SARS病毒S2蛋白的基因 ,并在原核表达系统中表达了 6×His S2硫氧还原融合蛋白。用Westernblot分析纯化Trx S2融合蛋白 ,分别与 6份SARS流行前的正常人血清和 6份SARS患者恢复期血清的反应性。结果 :Westernblot分析表明 ,6份SARS患者的恢复期血清均能识别Trx S2融合蛋白 ,且在Mr为 76× 10 3 附近出现特异性的结合带 ;而 6份SARS流行前的正常人血清则不与Trx S2融合蛋白起反应。结论 :本研究获得了纯化的SARS病毒Trx S2融合蛋白 ,它可与SARS患者的恢复期血清产生特异性结合反应 ,为研究SARS病毒感染宿主细胞的过程和制备针对SARS病毒的重组疫苗提供了条件。更多还原

【Abstract】 AIM: To express S2 protein of SARS virus fused with Trx and then detect its reactivity to the sera from convalescent SARS patients. METHODS: The Trx S2 fusion protein was expressed in E.coli . After purification, the Trx S2 fusion protein was detected by Western blot with 6 serum samples of convalescent SARS patients and 6 serum samples of healthy donors. RESULTS: According to the SDS PAGE analysis , the relative molecular mass ( M r) of the Trx S2 fusion protein is about 76×10 3. The fusion protein could react with all the sera from convalescent SARS patients but not with the sera from healthy donors. CONCLUSION: The Trx S2 fusion protein provides a basis for the research on its role in the course of SARS virus infection of host cells and preparation of recombinant viccine against SARS virus.更多还原