【摘要】 目的 :研究灵芝孢子粉碱提多糖 (LZSBS)对小鼠巨噬细胞的激活作用。方法 :用灵芝孢子粉碱提多糖刺激体外培养的小鼠巨噬细胞 ,用ELISA法检测巨噬细胞分泌至培养基中的TNF α和IL 1β的含量 ;用Griess法检测培养上清中NO的含量。小鼠巨噬细胞对乳胶颗粒的吞噬率用显微镜计数。结果 :经灵芝孢子粉碱提多糖刺激后 ,小鼠巨噬细胞变大 ,颜色加深 ,并能显著刺激巨噬细胞分泌TNF α和IL 1β ,并产生大量的NO。小鼠巨噬细胞对乳胶颗粒的吞噬功能也明显的增强。结论 :灵芝孢子粉碱提多糖对小鼠巨噬细胞具有明显的激活作用更多还原

【Abstract】 AIM: To investigate the activation of mouse macrophages by the alkali-e xtracted polysaccharides from the spore of Ganoderma lucidum (LZSBS). METHODS: The mouse macrophages cu ltured in vitro were stimulated by LZSBS. IL-1β and TNF-α in the culture supernatants were detected by EL ISA. NO production was detected by Griess assay. The percentage of phagocytosis of latex beads by mouse macrophages was counted under microscope. RESULT S: The mouse macrophages stimulated by LZSBS increased in volume and da rkened in appearance under phase-contrast microscope. LZSBS-activated mouse m acrophages secreted IL-1β and TNF-α produced a large amount of NO. The perc entage of phagocytosis of latex beads by mouse macrophages was also significantl y increased in the presence of LZSBS. CONCLUSION: LZSBS can acti vate markedly the mouse macrophages.更多还原