【摘要】 目的　调查 15个STR基因座 (D3S135 8、vWA、FGA、TH0 1、TPOX、CSF1PO、D5S818、D13S317、D7S82 0、D16S5 39、D8S1179、D2 1S11、D18S5 1、D2S1338、D19S4 33)在陕西省汉中地区汉族人群中的多态性分布 ,为群体学研究、法医学个体识别和亲权鉴定提供基础数据。方法　应用AmpF/STRIdentifiler荧光标记复合扩增试剂盒 ,结合ABI310 0Avant遗传分析仪 ,对汉中地区 10 3名汉族无关个体的静脉血进行扩增和电泳检测 ,并利用Genescan和Genotype软件进行自动分型。结果　 15个STR基因座在汉中地区汉族人群中的等位基因频率分布在 0 .0 0 4 9～ 0 .5 0 4 9,基因型频率分布在 0 .0 0 97～ 0 .32 0 4 ,累计耦合概率为 5 .5× 10 -17,累计非父排除率为 0 .999999999。结论　我们所应用的 15个STR基因座在陕西汉中地区汉族人群中具有较高的多态性 ,适合于该地区的群体学研究、法医学实践更多还原

【Abstract】 Objective To investigate the polymorphism of 15 short tandem repeat(STR) loci of Han population in Hanzhong area, Shaanxi Province to provide basic data for population study, forensic cases and parentage testing. Methods Blood samples were obtained from 103 unrelated Han population from Hanzhong. DNA templates were screened using AmpF/STR Identifiler kit and ABI3100Avant DNA analyzer with Genescan and Genotype software. Results The allele frequencies of 15 STR loci ranged from 0.004 9 to 0.504 9, and genotype frequencies ranged from 0.009 7 to 0.320 4. The combined match probability was 5.5×10 -17 and combined EPP was 0.999 999 999. Conclusion The 15 STR loci used in this study are highly polymorphic in Han population from Hanzhong area and can be applied to population study and forensic practice in this region.更多还原