【摘要】 用测定净光合放氧速率的方法研究了温度、光照和pH对锥状斯氏藻(Scrippsiellatrochoidea)和塔玛亚历山大藻(Alexandriumtamarense)光合作用的影响。在不同光暗周期(L:D)条件下培养锥状斯氏藻和塔玛亚历山大藻,研究了光暗周期对生长繁殖速率和生物量的影响。2种藻对温度的变化敏感,适宜的温度范围是17～25℃,最适温度20～22℃,低于10℃和高于30℃不能生长;锥状斯氏藻的光饱和点是400μmol·m-2·s-1,塔玛亚历山大藻的光饱和点是650μmol·m-2·s-1,都属于喜高光强的微藻;2种藻对pH值的变化极其敏感,适宜的pH值范围很小,为7.0～9.0,最适pH值7.5～8.0,与其生活的海洋环境一致,pH值高于9.5时,不能进行有效的光合作用,pH值10.0可致全部细胞死亡;在一定范围内,2种藻的生长速率(μ)和生物量随着光照时间的延长呈比例增加。更多还原

【Abstract】 The effects of temperature, light intensity and pH on photosynthesis of Scrippsiella trochoidea and Alexandrium tamarense were investigated. The effects of light-dark cycle on growth rate and biomass were investigated by cultivation of the two algae under different photo phases. These two algae were sensitive to temperature, suitable temperature 17-25℃, and the optimal temperature 20-22℃. They could not grow when the temperature was below 10℃ or above 30℃. The saturation light intensity for S. trochoidea and A. tamarense were 400 μmol·m^-2·s^-1 and 650 μmol·m^-2·s^-1, respectively, both of them were adaptable to high light intensity. These two algae were very sensitive to pH, suitable pH 7.0-9.0, and the optimal pH 7.5-8.0, which were accordant to that of the sea water. The photosynthesis of these two algae were not effective when pH was above 9.5, pH10.0 was deadly to them. Growth rate and biomass increased with the extension of photo phase in certain range.更多还原