【摘要】 甘油脱水酶是1,3 丙二醇发酵过程中的重要限速酶,具有很好的工业应用前景.其中α亚基为甘油脱水酶的主要组成部分,该基因的正确克隆与分析对甘油脱水酶的正确表达起重要作用.作者采用PCR技术,从肺炎克雷伯氏杆菌A.S.1.1736基因组中扩增得到了编码甘油脱水酶α亚基的gldA基因,并将其克隆至pMD18 T载体中.经核苷酸序列分析证实,gldA基因开放阅读框架由1668bp组成.经GenBank检索对照分析,gldA基因序列与国外文献报道的同源性达99.34%,表明所克隆的gldA基因即为克雷伯氏甘油脱水酶α亚基基因.更多还原

【Abstract】 The glycerol dehydratase catalyzes the rate-limiting step in the anaerobic conversion of glycerol to 1,3-propanediol and is comprised of three different subunits. The gldA gene coding glycerol dehydratase α subunit has an important role in the expression of glycerol dehydratase . In our study, the gldA gene was amplified by PCR using the genomic DNA of Klebsiella pneumoniae as the template, and then was cloned into vector pMD18-T. After sequencing, it was found that, the gldA gene consists of 1 668 bp encoding 555 amino acid residues. Through computer analysis, the gene was known to be consistent with the abroad reported gldA gene up to 99.34%.更多还原