【摘要】 介绍了一种基于MEMS技术的PDMS电泳微芯片,该芯片主要由三部分组成:集成了高压电极的玻璃基底、PDMS薄膜以及刻有微流路的PDMS板。利用lift off工艺,在玻璃基底上制作了为电泳分离提供高压的Pt电极。为了提高PDMS微芯片的密封效率,同时也为了保持微流路材料的一致性,通过挤压法首先在玻璃基底上形成了一层PDMS薄膜。电泳分离实验表明:在该微芯片上能够实现DNA片段的有效分离。更多还原

【Abstract】 The fabrication of PDMS electrophoresis microchip based on MEMS technology is reported.The microchip is composed of glass substrate with integrated electrodes,a PDMS thin membrane and PDMS wafer with microchannel network.Pt electrodes,used to offer high electric field for driving electrophoresis separation,are fabricated on the glass wafer using lift-off process.To improve the sealing of PDMS microchip and ensure the uniformity of microchannel material,a PDMS membrane is first formed on glass substrate by pressing method.The experiments demonstrate that DNA separation can successfully achieved on this microchip.更多还原