【摘要】 为了配合研究利什曼原虫 (Leishmania)的金属抗药性 ,本文建立了一种氢化物发生 -原子荧光法测定细胞内痕量锑的方法。在选定最佳仪器条件下测定 ,锑的线性范围达到 0～ 2 0 μg L ,检出限为 0 0 8μg L ,加标回收率为 92 8% - 10 3 6 %。此方法操作简便、灵敏度高、费用低廉。所获结果与电感耦合等离子体质谱 (ICP MS)测试结果进行了比较 ,经配对t检验t=0 0 0 1<t0 0 5,10 =2 2 2 8(P >0 0 5 ) ,两者无显著性差异。该方法已应用于寄生虫的金属抗药性研究 ,获得满意的结果。更多还原

【Abstract】 An new and simple method to determine the antimony (Sb) in cells using Hydride Generation-Atomic Fluorescence Spectrometry (HG-AFS) was established for studying the mechanism of metal resistance in the protozoan parasite Leishmania. The detection limit was 0.08μg/L respectively. The average recoveries at different concentrations were 92.8%-103.6%. This method was simple, precise,reliable. The obtained results were compared with those determined by ICP-MS, and no obvious differences could be observed. This method has been successfully applied to the mechanism study of metal resistance in Leishmania.更多还原