【摘要】 以表面消毒法分离白肋烟TN90和TRM品种 30个样品的内生细菌 ,共分离到 33株内生细菌。对这些菌株进行了初步分类鉴定 ,它们属于假单胞菌属、黄单胞菌属、节杆菌属、葡萄球菌属、棒杆菌属、黄杆菌属、气球菌属和利斯特氏菌属。筛选出TEB1 1、TEB1 7、TEB2 3、TEB2 6、TEB30、TEB34等 6株还原硝酸盐和亚硝酸盐能力较强的菌株 ,以粉碎烟叶接种、叶柄浸泡接种和叶面喷雾接种 3种方式处理调制后 ,化验分析结果表明 ,接种内生细菌能降低2 7 5 6 %～ 99 88%白肋烟TSNA含量 ,降低百分率以粉碎烟叶接种最高 ,其次是叶柄浸泡接种 ,叶面喷雾接种最低更多还原

【Abstract】 By surface disinfection method, thirty three strains of endophytic bacteria were isolated from the midrib of thirty samples, including burley tobacco TN90 and TRM. These strains were identified as eight genera, they are Pseudomonas, Xanthomonas, Arthrobacter, Staphylococcus, Corynebacterium, Flavobacterium, Rhizobium, Aerococcus, and Listeria. The strains TEB11、TEB17、TEB23、TEB26、TEB30 and TEB34 were screened by nitrate and nitrite reducing test, and inoculated to burley tobacco leave by three methods: homogenized leave, dipped stalks and sprayed leave surface. The contents of NO 3N, NO 2N, TSNA and alkaloid of these samples cured were analyzed. The results showed that Endophytic bacteria could reduce 27 56%～99 88% of the TSNA contents of burley tobacco leave, the TSNA contents of homogenized leave inoculated were lowest, then were dipped stalks and sprayed leave surface were highest.更多还原