【摘要】 通过硫酸铵分级沉淀 ,阴离子交换层析 ,凝胶过滤 3步从嗜碱菌Bacillussp .ZBAW6纯化了木聚糖酶。结果表明该酶分子量为 4 5kD。N末端序列为DPFAAAVAPL。在pH5 5～ 1 0 5范围内均具有较高酶活性和稳定性 ;最适反应温度为 6 5℃ ,酶活力基本不变。该酶作用于Beech xylan的Km 为 0 1 1mg mL ,Vmax 为 2 3 89μmol (min·mg)。Hg2 + 对该酶有强的抑制作用更多还原

【Abstract】 A xylanase produced by Bacillus sp. ZBAW6 was purified to electrophoretic homogeneity by three steps including (NH 4) 2SO 4, Cellulose DE 52 and Bio gel P100. Its molecular weight is around 45kD by SDS PAGE. Its N terminal sequence was DPFAAAVAPL. The optimal pH and temperatuer of the enzyme activity were 5 5～10 0 and 65℃. Mcihaelis constant of the enzyme was 0 11mg/mL and V max=23 89μmol/(min·mg) with beech xylan as the substrate.The activity was completely inhibited by Hg 2+ .更多还原