节点文献
含谷胱甘肽硫转移酶基因工程菌表达条件研究
The Research of Gene Expression Condition of the Glutathione S-TransferaseIn Escherichia coli BL21(DE3)
【摘要】 将重组谷胱甘肽硫转移酶 (GST)大肠杆菌表达株BL2 1(DE3 )PGEX 作为研究对象 ,进行了生长及表达条件的优化研究 ,分析比较了不同培养条件对菌体生长的影响以及诱导剂的添加量对产物表达量的影响。
【Abstract】 Regarded the recombinant Glutathione S-Transferase in Escherichia coli BL-{21}(DE-3) as target. We investigated the growth condition and expression condition of E.coli BL-{21}(DE-3)P+{GEX}.The influences of different culture conditions on cell growth and inducement to expression product was analyzed and studied.
- 【文献出处】 微生物学杂志 ,Journal of Microbiology , 编辑部邮箱 ,2004年04期
- 【分类号】Q814
- 【被引频次】3
- 【下载频次】143