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玉米和花生根际生物的原位观察初探
IN-SITU OBSERVATION OF ORGANISMS IN THE RHIZOSPHER OF MAIZE AND PEANUT USING IMPROVED ROOT BOX METHOD
【摘要】 利用改进根箱法对玉米和花生根际生物进行了原位采样和观察。采用载玻片贴片方法直接采集到作物根系分泌物中的生物 ,并用荧光显微镜进行观察。花生根系分泌物中分布有微生物 ,但是根系表面未发现生物 ;玉米根系分泌物中分布有线虫和生物体 ,根系表面分布有生物体 ,其形态与根系分泌物中生物体形态相似。表明此方法是一种原位研究根际生物的较为有效的方法。
【Abstract】 The organisms from crop root exudates were collected directly by putting slide onto root surface and observed the organisms on the slide and root surface by fluorescence microscope. It was showed that microorganisms were distributed on peanut root exudates but no organism was found on root surface. Nematode and unidentified organisms were seen on the slide from maize root exudates, while similar organisms were found on maize root surface. This indicated that this is a useful method for in situ observation of rhizospheric organisms.
【基金】 国家自然科学基金重大项目 (No 3 9790 1 0 1 );国家重点基础研究发展计划 ( 973计划 )项目资助 (No 2 0 0 2CB41 0 81 0 )
- 【文献出处】 微生物学通报 ,Microbiology , 编辑部邮箱 ,2004年01期
- 【分类号】S154
- 【被引频次】4
- 【下载频次】359