【摘要】 以DMEM∶F12(1∶1)为基础培养基,通过观察细胞生长状态和检测乙肝表面抗原的表达量作为评价指标,筛选适合于CHO工程细胞生长的生长因子,如胰岛素、转铁蛋白、氢化可的松、硒酸钠,丁二胺等。并且建立了J5SFM培养基。该培养基与商品化的无血清培养基比较,能够使细胞生长维持较长的时间,表达产物分泌量也相对较高。更多还原

【Abstract】 The J 5 serum free medium is established based on DMEM∶F12 (1∶1),and it is developed by adding the growth factor such as insulin, transfferin, hydrocortisine, sodium selenate putrescine etc. Its suitability is valuated by observing the growth situation of CHO cell and detecting the expression level of HBsAg. In comparison with commercial SFM,it can maintain growth of CHO cell for much longer peroid and the expressing level of HBsAg is higher too.更多还原