【摘要】 采用平均分子量为 5kD的已活化的单甲氧基聚乙二醇 (mPEG)对重组人 β 干扰素 (rhIFN β)进行化学修饰。优化修饰条件 ,选择对rhIFN β反应性高的修饰剂 ,制备三种不同修饰程度的修饰物 ,并对修饰物进行初步检定。三种修饰物的修饰率分别为 10 .12 %、2 2 .99%、4 2 .4 7% ;比活性分别保留为原来的 93.5 3%、4 8.6 9%、13.18% ;PEG修饰后的rhIFN β在pH7时的溶解度增加更多还原

【Abstract】 rhIFN-β was modified by activated methoxypolyethylene glycol (mPEG). With the optimization of modifying conditions, CC-mPEG was chosed to modify rhIFN-β and three peglated rhIFN-β were prepared,the extents of modification are 10.12%, 22.99%, and 42.47% respectively. Specific activity of three peglated rhIFN-β is 93.53%, 48.69%, 13.18% of original rhIFN-β respectively. Polypeglation had effect on the configuration of rhIFN-β. The peglation increased the solubility of rhIFN-β at neutral pH.更多还原