【摘要】 采用正交设计法研究了细胞分裂素ZT,生长素NAA,培养基pH值和光照强度对洋桔梗叶盘不定芽诱导的影响.结果表明,实验的4个因子对洋桔梗叶盘不定芽都具有极显著的诱导作用,最佳培养基和培养条件为:MS+ZT 0.5 mg/L+NAA 0.01 mg/L,培养基pH值为6.3,光照强度为3000 lx.该处理中每叶盘再生不定芽数平均达9.41个.更多还原

【Abstract】 The effects of ZT, NAA, pH value and the intensity of illumination on the bud induction of Eustoma rus-sellianum have been studied by L9(34) orthogonal design. Results of the various analysis show that the four factors all have extremely remarkable effects on the bud induction. The best culture media and conditions for the bud induction of Eustoma russellianum are MS + ZT 0.5 mg/L+ NAA 0.01 mg/L, pH value of medium is 6.3, the intensity of illumination is 3000 lx.Under these conditions, 9.41 buds have regenerated from every leaf disc.更多还原