【摘要】 以骨组织工程学的方法制备了一种新型BMP/α-TCP复合骨水泥材料.通过体外蛋白质释放实验、强度实验及X射线衍射分析,考察了BMP在复合材料中的释放规律;通过组织学分析和X射线检查,研究了新型复合骨水泥材料对兔颅骨缺损的修复实验.结果表明:在模拟体内环境中,BMP自BMP/α-TCP复合材料中的释放规律符合Higuchi方程,BMP以扩散方式释放,BMF和α-TCP骨水泥复合后固化时间不受影响,BMP释放后材料强度随浸泡时间延长而增高,BMP释放4周后强度达到纯α-TCP骨水泥固化强度,X射线衍射分析表明材料向羟基磷灰石转化.骨缺损修复实验表明:新型骨水泥成骨能力强,能在短期内完全修复兔颅骨缺损.更多还原

【Abstract】 A novel BMP/α-TCP composite bone cement was developed by bone tissue engineering approach. The releasing behavior of BMP from the composite was investigated in vitro through protein releasing testing, compressive strength measurement and XRD analysis. With the help of histological study and X radiographic examination, the skull defect of rabbit was repaired by the novel composite bone cement, and α-TCP bone cement was discussed as the control. Results in simulate body environment show that the rules of BMP releasing from BMP/α-TCP composite agree with Higuchi equation, which indicate that BMP releasing accords with diffusion model. The setting time of the bone cement is not influenced by the combination of BMP and α-TCP. After BMP releasing, the compressive strength of materials increases with time, and the value reaches the level hardened pure α-TCP bone cement at fourth week. The crystalline phase of materials is transferred into hydroxyapatite (HA) with time increasing. Animal experiments confirm the strong osteogenesis capacity of the novel bone cement. Rabbit skull defect can be well healed with the materials in short period.更多还原