【摘要】 以聚苯乙烯小球为固相载体,在小球表面包被一层基因工程制备的乙型肝炎核心抗原(HBcAg),以125I标记的乙型肝炎核心抗体(125I-Anti-HBc)作为标记物,采用固相放射免疫竞争法研制成乙型肝炎核心抗体放射免疫定量试剂盒(Anti-HBc-RIA)。本试剂盒的灵敏度为0.8NCU/mL,批内变异系数<9.9%,批间变异系数<13.8%,回收率为92.6%～109.2%。正常参考值为0～2.0NCU/mL。更多还原

【Abstract】 A sensitive and specific solid phase radioimmunoassay for hepatitis B core antibody（anti-HBc-RIA） is established. Hepatitis B core antigen（HBcAg） is coated on the solid phase beads, ¹²⁵I-anti-HBc is used as the radioactive tracer. The sensitivity of the assay is 0.8 NCU/mL. The intra-assay CVs and the intre-assay CVs of 3 samples are 9.9% and 13.8% respectively. The analytical recoveries are ranged from 92.6% to 109.2%. The level of 120 normal subjects is 1.2 NCU/mL with a rang of 0².0 NCU/mL. The method for measuring anti-HBc in human serum is rapid, sensitive, convenient and inexpensive. It is suitable for clinical and research application.更多还原