【摘要】 在以钾长石粉为唯一 K 源的硅酸盐细菌选择性培养基上,从我国部分省市土壤中筛选到 16 株硅酸盐细菌,以本实验室保藏 NBT 菌株为参照,对其生理生化特性、耐盐性、抗生素抗性、温度敏感性及释K 能力等生物学特性进行了测定。结果表明,17 株硅酸盐细菌菌体均为杆状,产生椭圆至圆形芽胞。其中 SB6、SB13 为短杆状,SB4、SB6、SB10、SB15 菌株是 G+,其余菌株是 G-。NH4 、NO3 为良好 N 源,且能在无 N + -培养基上生长。菌株 SB13 和 NBT 解 K 能力较强,释放的 K 比接灭活菌对照分别增加 49.1 %和 45.3 %。菌株SB2、SB4、SB5、SB6 在 20 g/L NaCl 浓度的培养基上能生长,在温度为 10 ~ 40 范围内供试硅酸盐细菌能够良好生长。更多还原

【Abstract】 Cultured on selective medium based on feldspar as its sole potassium source, 17 strains of silicate-dissolving bacteria were screened out of soils sampled from several provinces in China. Their physiological and biochemical characters, salt tolerance, antibiotic resistance, temperature sensitivity and K releasing capability were determined. The results show that all strains appear as rods and yield ellipsoidal spores, but Strains SB6 and SB13 are shorter. The Gram reaction of Strains SB4, SB6, SB10 and SB15 were positive and the others negative. The isolates grew well with NH4 and NO3 as nitrogen source and survived without. The strains were compared in capability of + - releasing potassium and it was found that Strain SB13 and NBT were more capable of releasing potassium. The content of soluble potassium was increased by 49.1% and 45.3%, respectively. Strains SB2, SB4, SB5 and SB6 survived in the medium with 20.0 g/L NaCl and were growing well under the temperature ranging from 10℃~40℃.更多还原