【摘要】 目的　检测肾移植患者外周血淋巴细胞亚群、单个核细胞凋亡情况 ,结合临床诊断及临床表现 ,探讨肾移植患者手术前后淋巴细胞亚群、外周血单个核细胞凋亡及免疫抑制治疗的关系。方法　收集 2例移植患者术前2天 ,术后 2天 ,8天 ,16天外周血 ,利用流式细胞仪进行淋巴亚群及PBMC凋亡检测。结果　与正常参考值相比 ,两例肾移植患者CD3+细胞百分率 (6 1.5 4～ 73.14 ) %在正常范围 ,CD3- CD19+ 细胞百分率较高 (11.14～ 2 9.0 6 ) % ,CD3- CD16 +5 6 + 细胞百分率 (3.5 4～ 2 5 .5 0 ) %较低 ;CD4 + 细胞百分率逐渐增加 (30 .4 8～ 4 1.0 6 ) % ;CD8+ 细胞百分率 (2 6 .5 5～ 34.0 8) %则逐渐降低 (31.74～ 2 7.0 2 ) % ;CD4 CD8比值 (0 .96～ 1.5 2 )逐渐升高 ;PBMC凋亡率术前 2天分别为 10 .2 6 %、8.2 8% ,术后 2天分别为 14 .32 %、11.75 %、术后 8天分别为 12 .93%、8.4 2 % ,术后 16天分别为 9.13%、5 .76 %。结论 :在免疫抑制剂与患者免疫系统的综合作用下 ,肾移植患者术后 2天和术后 8天PB MC细胞凋亡率较高 ;CD4 CD8比率控制在正常范围内更多还原

【Abstract】 Objective: To study the relationship among lymphocyte subsets、peripheral mononuclear cells (PBMC) apoptosis and immunosuppressant by examine PBMC apoptosis and lymphocyte subsets in kidney transplant recipients. Methods: Heparin treated peripheral bloods were collected from 2 kidney-transplanted patients, PBMC apoptosis and lymphocyte subsets were tested by flow cytometry (FCM). Results: The percentage of CD3~+ cells(61.54～73.14)% was within the normal range; CD3~-CD19~+ cells (11.14～29.06)% and CD4~+ cells (30.48～41.06)% were higher than normal range, while CD3~-CD16+56~+ (3.54～25.50)% and CD8~+ cells (26.55～34.08)% were lower. The ratio of CD4+ cells and CD8+ cells became higher. The apoptosis percentage of PBMC in two patients was different, but both of rates were higher in day 2 and day 8 post-transplant, and were lower in day 2 pre-transplant and day 16 post-transplant. Conclusion: PBMC apoptosis were high in day 2 and day 8 post-transplant, and CD4~+/CD8~+ ratio was improved under Cyclosporin、Prednisone and Azathioprine treatment.更多还原