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大孔吸附树脂对紫锥菊提取物中菊苣酸分离纯化的研究
STUDY ON ADSORPTION AND PURIFICATION OF CICHORIC ACID IN EXTRACTS OF ECHINACEA PURPUREA WITH MACROPOROUS ADSORPTION RESIN
【摘要】 采用大孔吸附树脂分离纯化紫锥菊提取物中免疫活性成分菊苣酸 ,7% (v/v)的甲醇 水溶液洗脱 ,HPLC法对产品中菊苣酸含量进行检测分析。该法能把紫锥菊提取物中菊苣酸含量 (4 % )提高 9倍左右 ,回收率达到 95 %以上 ,且操作简单 ,可用于菊苣酸的分离纯化。
【Abstract】 Developed a method to separate and enrich the immunocompetence constituent cichoric acid from the extracts of Echinacea purpurea with macroporous adsorption resin,by eluting with 7% (v/v) CH 3OH-H 2O. Cichoric acid content in product quantified by HPLC was nine times higher than that (4%) of the extracts of Echinacea purpurea. The recovery of cichoric acid was more than 95%. The technology was simple and could be applied to separate and purify cichoric acid.
【关键词】 大孔吸附树脂;
紫锥菊提取物;
菊苣酸;
【Key words】 macroporous adsorption resin; extracts of Echinacea purpurea; cichoric acid;
【Key words】 macroporous adsorption resin; extracts of Echinacea purpurea; cichoric acid;
【基金】 国家“十五”攻关项目 ( 2 0 0 1BA746C) ;湖南省重点项目 ( 2 0 0 1BA80 4A2 1) ;湖南省自然科学基金( 0 2JJY2 0 2 3 )
- 【文献出处】 天然产物研究与开发 ,Natural Product Research and Development , 编辑部邮箱 ,2004年02期
- 【分类号】O658;O657.7
- 【被引频次】27
- 【下载频次】569